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產品
Phospho-Threonine Antibody產品詳細說明書:
  • 價格:¥2950
  • 類 型:Phosphorylation
  • 包 裝:100 μg
  • 發布時間:2018-1-5 9:40:29
關于 Phospho-Threonine Antibody 的詳細介紹

Catalog #

Product Description

ICP9807

Affinity purified rabbit polyclonal phospho-threonine antibody (anti-pT) was developed using a phospho-threonine-KLH conjugate and phosvitin mixture as the immunogen. The phospho-threonine specific antibody was affinity purified with phospho-threonine immobilized on agarose.

Detection of a phosphorylated protein kinase with anti-phosphothreonine (ICP9807)                                                      Lane1-2:  Untreated kinase                          Lane 3-4: Kinase treated with  phosphatase PP1G
Western blot analysis of mouse extract spleen with ICP9807                                      A: Extract of spleen from mouse treated  with sodium vanadate                                  B: Extract of spleen from control mouse
Species
Rabbit
Formulation
250 μg/mL in PBS, pH 7, with 50% glycerol
Immunogen
Phospho-threonine KLH conjugates and phosvitin mixture
Purification
The antibody was affinity purified with phospho-threonine on agarose followed by affinity absorption with threonine on agarose.
Specificity
The antibody recognizes proteins and peptides phosphorylated on threonine residues. There are no cross reactions with phospho-tyrosine.
Species Tested
Human and mouse
Applications
ELISA; WB; IP
Scientific Description
Threonine is one of the 20 naturally occurring amino acids. Kinases phosphorylate threonine to phospho-threonine which enters various biosynthetic pathways in the body.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.
References
1. M.J. Betts, R.B. Russell. Bioinformatics for Geneticists, M.R. Barnes, I.C. Gray eds, Wiley, 2003.

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